Omics · study · 2026
scRNA-seq of limb-mesenhymal organoid derived from mouse embryo during digit-like morphogenesis
Listed in NCBI GEO
We performed single-cell RNA sequencing of mouse embryonic limb mesenchymal cells and limb mesenchymal organoids during digit-like morphogenesis.
Description
Distal and proximal limb mesenchymal cells were isolated from E11.5 Slc:ICR mouse embryos, labeled using lipid-DNA CellPlex/Feature Barcode oligonucleotides, and profiled either immediately as Day0 samples or after culture as limb mesenchymal organoids under serum-only control or Fgf8b/Wnt3a-supplemented conditions.
The dataset includes Day0 distal and proximal cells, Day1 and Day2 control organoids, and Day1 and Day2 Fgf8b/Wnt3a-treated organoids. These data were used to characterize distal and proximal cell states, treatment-dependent transcriptional changes, and lineage/state trajectories associated with self-organized digit-like morphogenesis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE331nnn/GSE331455 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE331455 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1468166 ncbi.nlm.nih.gov/bioproject/PRJNA1468166 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE331455 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |