Omics · study · 2026
Single-cell RNA sequencing data indicate that CD40 promots macrophage efferocytosis following myocardial infarction.
Listed in NCBI GEO
CD40 expressed on the macrophage membrane is recognized as one of the characteristic biomarkers for their inflammatory phenotype.
Description
We demonstrated that macrophage CD40 functionally promotes post-myocardial infarction (MI) repair. To elucidate the role of CD40 in the post-MI repair process, we dissected infarct and border zones from the hearts of wild-type (WT) and CD40 knockout (KO) mice at 3 days post-MI.
Mononuclear macrophages (CD45⁺CD11b⁺CD64⁺) were isolated via flow cytometry and subjected to single-cell RNA sequencing analysis. This study establishes that CD40 plays a critical role in macrophage efferocytosis. Furthermore, analysis of scRNA-seq data led to the identification of developmental precursor cells of repair-phenotype macrophages.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE322nnn/GSE322626 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE322626 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1430736 ncbi.nlm.nih.gov/bioproject/PRJNA1430736 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Myocardial infarction 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE322626 | 6 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:myocardial-infarction | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |