Omics · study · 2026
Single-cell RNA-seq reveals the keratinocyte heterogeneity and increased interfollicular basal keratinocytes in wound-induced hair follicle neogenesis
Listed in NCBI GEO
Scarring alopecia is an unresolved challenge characterized by scar formation following skin injury without hair follicle (HF) regeneration.
Description
Wound-induced hair follicle neogenesis (WIHN) is a model in which HFs regenerate after skin wounding. This study aimed to explore the mechanisms underlying WIHN, hinting for the new therapeutic strategies for scarring alopecia.
Our results indicated that keratinocytes were categorized into 8 subpopulations, with a notable increase in the proportion of INFU basal KCs in tissue with WIHN, which was validated by IF staining. Pseudotime analysis revealed that the marker gene Fst of INFU basal KC was activated earlier in tissue with WIHN. Fibroblasts were classified into 5 subpopulations, with an increase in the proportions of secretory reticular fibroblasts.
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Cell-cell communication analysis showed that interactions between INFU basal KC and secretory reticular fibroblasts were significantly enhanced. Enrichment analysis further demonstrated that extracellular matrix (ECM)-receptor interactions were significantly enriched in ligand-receptor interactions. Masson staining exhibited decreased collagen content in tissue with WIHN.These findings suggest that the importance of INFU basal KCs in WIHN and that the ECM reconstruction is crucial for WIHN.
Our study contributes to a better understanding of the underlying mechanisms of WIHN.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE308nnn/GSE308939 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE308939 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1333457 ncbi.nlm.nih.gov/bioproject/PRJNA1333457 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE308939 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |