Raw targeted free fatty acid metabolomics data from hepatocyte-specific ROMO1-knockout, ROMO1-overexpressing, and control mice

ROMO1_hOE_FFA_raw_data.zip corresponds to the overexpression cohort, with FFA_Control_1 through FFA_Control_7 and FFA_hOE_1 through FFA_hOE_5. Raw data format: Waters MassLynx RAW directories.</p><p dir="ltr"><br></p><p dir="ltr">Sample preparation: Liver tissues were freshly collected following exsanguination, homogenized in ice-cold 80% methanol, subjected to four freeze-thaw cycles (liquid nitrogen for 1 min followed by thawing at 25 degrees C), and centrifuged at 15,000 x g for 10 min at 4 degrees C. Supernatants were collected for analysis.</p><p dir="ltr"><br></p><p dir="ltr">Chromatography: Waters ACQUITY UPLC I-Class system with a Waters CORTECS T3 C18 column (30 x 2.1 mm, 2.7 micrometers), at 60 degrees C. Mobile phase A was water with 0.01% formic acid and 0.2 mM ammonium formate; mobile phase B was 50% isopropanol/50% acetonitrile with the same additives.

Flow rate: 1.3 mL/min; injection volume: 2 microliters. The gradient increased from 50% to 98% B over 1.2 min, held at 98% B for 0.5 min, and returned to the initial composition for re-equilibration.</p><p dir="ltr"><br></p><p dir="ltr">Mass spectrometry: Waters Xevo TQ-S micro triple-quadrupole mass spectrometer with negative electrospray ionization and compound-specific pseudo-MRM transitions. FFA assignments used pseudo-MRM transitions and retention-time windows adapted from the Waters MetaboQuan-R application method.

Acquisition and processing software: MassLynx v4.2 SCN977.</p><p dir="ltr"><br></p><p dir="ltr">Each cohort reports 14 FFA species, but the panels differ. Shared species: FFA 22:4, 22:6, 20:4, 20:5, 18:0, 18:1, 18:2, 18:3, 16:0, 16:1, and 10:0. The knockout panel additionally reports FFA 20:1, 20:2, and 20:3; the overexpression panel additionally reports FFA 14:0, 8:0, and 6:0.

Lipid annotations are retained as carbon-number:double-bond-count labels.</p><p dir="ltr"><br></p><p dir="ltr">Related manuscript: A redox-coupled ROMO1-LONP1-SIRT3 axis controls hepatic lipid catabolism.</p>