Single-cell multiome data of 4 LOY clones and 4 ROY clones of A549 cell lines

Cells were incubated with the lysis buffer for 4 minutes. Single-nuclei ATAC and 3’ gene expression sequencing libraries were generated using the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle (10X Genomics) according to the manufacturer’s instructions.

Per well of the Chromium Next GEM Chip J (10X Genomics) one sample with 15,000 nuclei was loaded. Libraries were sequenced on a NovaSeq 6000 system using the S4 v1.5 flow cell with paired-end 200 cycles (1 lane for gene expression and 3 lanes for ATAC libraries). </p><p dir="ltr">Raw read data was processed using 10x Genomic’ Cellranger-ARC v2.0.2 software using default parameters to generate gene count matrices and fragment files for snRNA-seq and scATAC-seq, respectively.</p><p dir="ltr">The dataset contains the following layers:</p><p dir="ltr">RNA: scRNA-seq data of the 4 LOY and 4 ROY cell lines (metadata column: sample.name)</p><p dir="ltr">ATAC: scATAC-seq data for the same cells</p><p dir="ltr">Integration has been performed with Seurat's WNN method.</p>