Omics · study · 2026
Human Primordial Germ Cell Heterogeneity in vitro is Associated with Distinctive Biological States [WGBS]
Listed in NCBI GEO
Primordial germ cells (PGCs) exhibit molecular heterogeneity during development, but whether this reflects distinct biological states remains unclear.
Description
Using human PGC-like cells (hPGCLCs) in extended culture with dual fluorescent reporters for TFAP2C and DND1, we investigated the functional significance of DND1 expression heterogeneity. Single-cell RNA sequencing revealed DND1+ hPGCLCs are enriched in RNA regulatory genes including those associated with P-bodies and translational control.
DND1+ cells exhibited reduced proliferation, cell cycle exit, and elevated P27 (CDKN1B) expression compared to DND1- cells. Whole-genome bisulfite sequencing demonstrated enhanced DNA demethylation in DND1+ cells, particularly at gene bodies. DND1+ hPGCLCs showed stable expression of male germ cell differentiation markers including PIWIL1, PIWIL2, and DMRT1.
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BMP2 supplementation increased DND1 translation without affecting mRNA levels or proliferation rates. These findings demonstrate that DND1 marks a post-transcriptionally regulated developmental state in human germ cells, highlighting translational control mechanisms in germline development.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313386 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313386 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1379339 ncbi.nlm.nih.gov/bioproject/PRJNA1379339 ↗
project · from NCBI GEO
- PubMed 42343208 pubmed.ncbi.nlm.nih.gov/42343208 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Methylation profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- Genetics 70% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313386 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (70%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:methylation-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |