Omics · study · 2026
NGFR-marked basal duct progenitors drive ductal-acinar regeneration in injured salivary glands
Listed in NCBI GEO
Severe salivary gland injury causes long-term structural and functional impairment, yet the epithelial populations associated with repair remain incompletely defined.
Description
Here, we identify NGFR as a surface marker of a basal duct-associated epithelial population with organoid-forming capacity in the human salivary gland and show that Ngfr marks a conserved injury-responsive epithelial population in the mouse salivary gland.
Single-cell RNA sequencing of human salivary gland tissue revealed that NGFR was enriched in a restricted basal duct subpopulation distinct from differentiated epithelial and myoepithelial compartments. NGFR-positive cells exhibited a progenitor-like transcriptional profile and were enriched at early pseudotime positions in inferred ductal-acinar differentiation trajectories. Functionally, NGFR-positive cells isolated from human tissue and organoids showed enhanced organoid-forming capacity, and NGFR-enriched organoids engrafted into injured mouse salivary glands.
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In mouse salivary glands, Ngfr marked a rare epithelial population whose isolated Ngfr-positive fraction showed enriched organoid-forming activity and was detected in injury-associated ductal regions after duct ligation and local inflammatory injury. Lineage tracing using Ngfr-CreERT2; Rosa26-tdTomato mice further showed that Ngfr-lineage cells contribute to ductal and acinar epithelial compartments during post-injury regeneration.
Together, these findings define NGFR in human and Ngfr in mouse as conserved markers of an injury-responsive basal duct epithelial population that can be prospectively isolated and contributes to ductal and acinar epithelial compartments during post-injury salivary gland regeneration.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE330nnn/GSE330533 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE330533 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1464554 ncbi.nlm.nih.gov/bioproject/PRJNA1464554 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE330533 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |