Omics · study · 2026
Single-cell transcriptomic analysis of Wfdc2-deficient mouse lungs and response to amiloride treatment
Listed in NCBI GEO
This study investigated the transcriptomic consequences of WFDC2 deficiency in mouse lungs and the response to ENaC inhibition.
Description
WFDC2 deficiency caused respiratory distress associated with epithelial defects, immune activation, and hyperactivation of the epithelial sodium channel (ENaC). Single-cell RNA sequencing was used to profile lung cells from wild-type, Wfdc2 knockout, Wfdc2 p.C147W knock-in, and amiloride-treated mice.
The data reveal altered epithelial cell differentiation and increased immune cell populations in Wfdc2-deficient lungs, while amiloride treatment partially restored epithelial cell states and respiratory phenotypes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337641 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337641 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1490521 ncbi.nlm.nih.gov/bioproject/PRJNA1490521 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE337641 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |