Single-cell RNA-sequencing data for LUAD cancer patients

<p dir="ltr">5,000 CD45⁺ or CD45^neg sorted cells from cryopreserved distal normal lung or lung adenocarcinoma of 4 male donors were pooled and loaded per well of the Chromium Next GEM Chip G (10X Genomics) and loaded into the 10X Genomics Chromium controller according to the manufacturer’s protocol. Reverse transcription, cDNA amplification, and the subsequent library preparation from 250 ng of cDNA were performed following the 10X Genomics Single Cell 3′ gene expression v3 protocol. 10nM of the finished libraries were pooled and sequenced using Illumina NovaSeq 6000 instrument with an S4 flow cell, generating approximately 1,450 million 100bp paired-end reads.</p><p dir="ltr">The following layers are available:</p><p dir="ltr">RNA: single-cell RNA sequencing count table for 4 lung donors, sampling distal normal and tumor tissue (metadata column: Sample.type).

Two resolutions of cell typing are provided Cell.Type.L2 and the more fine-grained Cell.Type.L3.<br>integrated: Integrated layer across the 4 donors using Seurat's Canonical Correlation Analysis (CCA) method</p>