Analysis release: early-onset and late-onset colorectal cancer differ by a graded transcriptional shift rather than a discrete regulatory state

Between a quarter and seventy per cent of the module effects</p><p dir="ltr">disappear after adjustment for leukocyte, stromal and epithelial composition proxies. The cohort</p><p dir="ltr">contains no patient aged between 50 and 69 years, so every threshold between those ages yields an</p><p dir="ltr">identical partition and identical statistics, and a discrete boundary cannot be distinguished from</p><p dir="ltr">a continuous age gradient by this design.</p><p><br></p><p dir="ltr">Contents:</p><p dir="ltr">- analysis.py, figures.py, figures_supp.py - regenerate every number, table and figure in one pass</p><p dir="ltr">- rdata.py - minimal reader for R's RDX3 serialization format, so no R installation is required</p><p dir="ltr">- ols.py - ordinary least squares with HC3 robust covariance, and Benjamini-Hochberg correction</p><p dir="ltr">- data/ - inputs, with provenance and the original R analysis retained for reference</p><p dir="ltr">- results/ - all result tables, per-sample module scores, intermediate arrays and summary.json</p><p dir="ltr">- figures/ - main and supplementary figures at 300 dpi</p><p dir="ltr">- CHECKSUMS.txt, environment.txt - SHA-256 of every file, and interpreter and package versions</p><p><br></p><p dir="ltr">A single seed (20260916) governs all stochastic components.

Two negative controls are included</p><p dir="ltr">deliberately and reported in the manuscript because both came out negative: the group separation</p><p dir="ltr">on a t-SNE projection is reproducible across 200 seeds, and standardising features across the full</p><p dir="ltr">cohort before cross-validation changes the area under the curve by 0.0004.</p><p><br></p><p dir="ltr">Underlying data: NCBI Gene Expression Omnibus accession GSE213092.

No new data were generated.</p>