Data for: ALK R1275Q mutation drives expansion of SCP-like cells during sympathoadrenal commitment and primes neuroblastoma initiation

Methods

Human iPSCs (Control lines: Ctrl7, Ctrl10, Ctrl14; Patient lines: NB1, NB2, NB4) were differentiated into trunk neural crest cells (tNCCs), sympathoadrenal progenitors (SAPs), and mature sympathoadrenal cells (SAMs). Single-Cell RNA Sequencing (scRNA-seq): Platform: 10x Genomics Single Cell 3' v3.1. Sequencing: Performed on MGI DNBSEQ PE100.

Samples: Collected at four developmental stages: tNCC, SAP, Day 8 (D8), and Day 12 (D12) from Ctrl10, NB1, NB2, and NB4. Bulk RNA Sequencing: Platform: Illumina PE150 sequencing. Samples: Collected at six developmental stages: tNCC, SAP, Day 6, Day 8, Day 10 and Day 12 from Ctrl7, Ctrl10, Ctrl14, NB1, NB2, and NB4 File Content and Organization The dataset consists of the following file types: Raw Sequencing Data (FASTQ): Unprocessed sequencing reads in .fastq.gz format.

For scRNA-seq: Paired-end reads (e.g., R1 containing cell barcodes/UMIs, R2 containing the transcript insert). For Bulk RNA-seq: Paired-end reads (R1/R2). Filenames typically indicate sample ID, library (GE or HTO), lane number, and read direction (e.g., SampleID_GE/HTO_L001_R1_001.fastq.gz).

Processed Data: Gene Expression Matrices: Count matrices (e.g., .mtx, .tsv) generated by Cell Ranger (for scRNA-seq) or Salmon (for bulk RNA-seq). Metadata: Tables containing sample information and differentiation time points.