Raw targeted TCA cycle metabolomics data from hepatocyte-specific ROMO1-knockout, ROMO1-overexpressing, and control mice

<p dir="ltr">Targeted LC-MS/MS profiling of tricarboxylic acid (TCA) cycle metabolites in liver tissues from hepatocyte-specific ROMO1-knockout, ROMO1-overexpressing, and control mice. </p><p><br></p><p dir="ltr">The study comprises 21 male Mus musculus mice (129/SV, 16 weeks old): 4 controls and 5 ROMO1-knockout mice in the hKO cohort, and 7 controls and 5 ROMO1-overexpressing mice in the hOE cohort. Cohorts were analyzed in separate analytical batches using the same method.

No pharmacological or dietary treatment was administered.</p><p><br></p><p dir="ltr">Liver tissues were extracted with ice-cold 80% methanol, subjected to four freeze-thaw cycles, and centrifuged at 15,000 x g for 10 min at 4 degrees C. Analysis used a Waters ACQUITY UPLC I-Class system with an Agilent Poroshell 120 SB-Aq column and triple-quadrupole mass spectrometry in negative electrospray ionization, multiple-reaction monitoring mode.