Bile acid uptake activates STAT signaling and impairs natural killer cells in metabolic dysfunction–associated steatohepatitis

We aimed to assess the impact of bile acid concentration on NK cell functionality and identify the molecular and metabolic NK cell signaling pathways that influence liver fibrosis pathogenesis. To this end, we studied peripheral NK cells from patients with MASH, healthy donors, and a mouse model of MASH. Sodium+/taurocholate cotransporting polypeptide (NTCP) expression was 2.5-fold higher in peripheral blood NK cells from MASH patients with advanced liver fibrosis than in those from early liver fibrosis patients and healthy donors.

Greater taurocholic acid (TCA) uptake was observed in peripheral NKNTCP+ cells from the early and advanced liver fibrosis MASH patient groups than in NKNTCP- cells from the same patients. It was associated with increased levels of exhaustion markers and decreased activity. Moreover, single-cell RNA sequencing and functional analyses demonstrated that NTCP⁺ NK cells in advanced MASH adopt stress- and exhaustion-associated transcriptional programs with reduced translational activity, accompanied by increased STAT3 activation and diminished STAT1 signaling, highlighting their impaired cytotoxic function.

Following STAT3 inhibition, trNKNTCP+ cells presented significantly reduced NTCP expression, reduced NK cell exhaustion, and restored NK cell activity. In MASH mice, epigallocatechin gallate-mediated inhibition of NTCP expression reduced TCA uptake and attenuated liver fibrosis. Additionally, in immunodeficient liver fibrosis model mice, reconstitution with trNKNTCP- cells alleviated liver fibrosis, decreased proinflammatory and profibrotic cytokine levels, and ameliorated lipid homeostasis.

Our findings suggest that enhancing immune responses by restoring NK cell activity through NTCP antagonism may offer a clinically beneficial strategy to prevent liver fibrosis in patients with metabolic disorders, autoimmune diseases, and chronic viral hepatitis.