Omics · study · 2026
Single-cell RNA sequencing of iALI epithelial and mesenchymal cell derived from UHOMi003-A cell line
Listed in NCBI GEO
This study aims to specify the effect of air pollution (PM2.5 developed by NIST), on bronchial epithelium generated from induced pluripotent stem cells, called iALI.
Description
The exposition was performed during 15 days on basal side and media was changed everyday. We performed single-cell RNA-sequencing (10x Genomics Chromium) to capture 10 000 cells on EpCAM+ cells (epithelial cells) and on EpCAM- cells (mesenchymal cells), in both control and exposed condition.
Then, analyse was perfomed in R by using Seurat pipeline, to identified each cluster cells and induced and repressed genes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE242nnn/GSE242697 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE242697 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1014370 ncbi.nlm.nih.gov/bioproject/PRJNA1014370 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE242697 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |