Omics · study · 2026
The IL-10/IL-10Ra axis in fibroblasts limits large intestinal pathology by suppressing type I interferon signaling [scRNA-Seq, part2]
Listed in NCBI GEO
In the intestines, stromal cells are essential for epithelial homeostasis, supplying both stem cell niche signals and differentiation factors.
Description
Recent studies have shown that they play crucial roles not only in epithelial maintenance, but also in the immune system. However, the molecular mechanisms that control inflammatory response by stromal cells are poorly understood.
We showed that stromal cells from Rag2 knock out (KO) mice highly express inflammatory cytokines and chemokines, and the IL10-IL10R axis is a key suppressor for those inflammatory mediators in fibroblasts. Fibroblast-specific Il10ra KO mice developed spontaneous colitis in aged mice. We performed (sc)RNA-seq on stromal cells from these mice and 16s rDNA-seq on the feces from those mice.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306442 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306442 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1310833 ncbi.nlm.nih.gov/bioproject/PRJNA1310833 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE306442 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |