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Omics · study · 2026

Single-cell RNA sequencing of pulmonary myeloid cells from mixed bone marrow chimeric mice infected with Cryptococcus neoformans gcs1 mutant

Listed in NCBI GEO

scRNA-seq of sorted pulmonary myeloid cells (Live/CD45+/IV-/B220-/CD90.2-/Ly-6G-/MHCII+/CD11c+/CD64+) from mixed bone marrow chimeric mice at 35 days post-infection with C. neoformans gcs1 mutant.

Description

Chimeric mice were reconstituted with CD45.1 WT : CD45.2 WT (WT chimeras) or CD45.1 WT : CD45.2 STAT6-KO (STAT6-KO chimeras). Cells were labeled with hashtag antibodies (TotalSeq-C, BioLegend) and pooled for library preparation.

No sample was excluded due to quality issues.

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From keywords
Life Sciences
Inferred from text
RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
SourceKeyLast seenRaw
NCBI GEOGSE33119613 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[field].local:field:life-sciencesmapping · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencingsource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[method].geo_series_type:othersource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[modality].local:modality:rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
concepts[modality].local:modality:sequencingenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
concepts[modality].local:modality:single-cell-rna-seqenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
concepts[organism].NCBITaxon:10090source · NCBI GEOconnector:ncbi_geo@1.0.0/taxon
descriptionsource · NCBI GEOconnector:ncbi_geo@1.0.0/summary
publication_datesource · NCBI GEOconnector:ncbi_geo@1.0.0
titlesource · NCBI GEOconnector:ncbi_geo@1.0.0/title