Omics · study · 2026
Glandular Epithelium and Unexplained Recurrent Implantation Failure
Listed in NCBI GEO
Recurrent Implantation Failure (RIF), which refers to the failure to achieve clinical pregnancy following embryo transfer repetitively, continues to be a major obstacle in assisted reproductive technology.
Description
RIF could be caused by many disease and most of which is related to abnormal endometrium. However, unexplained RIF is a special situation which puzzles doctors and patients.
We use single-cell RNA sequencing to investigate the endometrial mechanism in unexplained RIF because the complicated environment of endometrium. Our results showed endometrium of RIF had higher levels of proliferation and activation of cytotoxic CD8+ T cells, which reject embryo implantation. Furthermore, glandular epithelium exhibits the highest metabolic activity, and it showed abnormal glycolysis and lower lactic acid production in the RIF, which was characterized by lower expression of genes related to glucose intake (SLC12A1), glucose metabolism (ALDOA), lactic acid production (LDHA) and output (SLC16A4) compared with the counterpart in the normal.
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The suppressive effect of lactic acid on CD8+ T cells was verified in vivo and vitro. We propose that RIF endometrium has not established the immune tolerance to embryo, which was attributed the abnormal glycolysis and lower lactic acid production in glandular epithelium.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE248nnn/GSE248784 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE248784 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1046008 ncbi.nlm.nih.gov/bioproject/PRJNA1046008 ↗
project · from NCBI GEO
- PubMed 41758694 pubmed.ncbi.nlm.nih.gov/41758694 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE248784 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |