Omics · study · 2026
CD4+ T cells in the developing human brain shape angiogenesis and neurogenesis
Listed in NCBI GEO
Although entry is limited, immune cells reside within and influence neural tissue function in health and disease.
Description
Unique T cell populations throughout the body may shape organogenesis, but these cells are absent during mouse embryogenesis. To investigate human prenatal T cell roles, we captured T cells from four midgestation organs: spleen, mesenteric lymph node, small intestine, and brain.
Single-cell RNA sequencing identified dominant CD4 populations and smaller subsets of cytotoxic CD8 and innate T cells. In the brain, distinct expression profiles were revealed, including a unique Trav1-2+ MAIT-like population. Spatial mapping showed T cells are predominantly localized within the ventricular angiogenic-neurogenic niche, adjacent to microglia.
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Functionally, co-culture of CD4 T cells and endothelial cells enhanced vascular network maturity, while transplanted CD4 T cells in brain organoids localized to the VZ niche and reduced cell death. Rag2 knock-out mice also show changes in brain vasculature and neuronal structure in the absence of B and T cells. These findings suggest T cells support tissue health and growth in the developing human brain and have implications for neurodevelopment in a growing population of surviving patients with immunodeficiency syndromes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300932 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300932 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1260576 ncbi.nlm.nih.gov/bioproject/PRJNA1260576 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Disease 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE300932 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |