Generation of alveolar organoids from human induced pluripotent stem cells using a stirred-tank bioreactor

We previously established a very simple and practical protocol for producing iPSC-LuOrgs from pre-differentiated embryoid bodies without the need of a gel-like extracellular matrix using a stirred bioreactor system.

Here, we investigated now an adapted protocol for the generation of iPSC-LuOrgs directly from iPSCs in stirred suspension and performed a comprehensive cellular and molecular characterization, including single-cell RNA sequencing, of the resulting LuOrgs differentiated in a bioreactor. Generated lung structures appeared to be less differentiated and were predominately of alveolar nature. Fibroblasts and bronchial epithelial cells were inefficiently generated.