Omics · study · 2026
Single-cell transcriptome sequencing approach reveals immunopathogenic responses in a murine model of Mycoplasma pneumoniae pneumonia
Listed in NCBI GEO
This study generated single-cell RNA sequencing (scRNA-seq) datasets of viable pulmonary CD45⁺ leukocytes isolated via FACS from male BALB/c mice (4–5 weeks old) under control conditions and at day 5 post-infection with Mycoplasma pneumoniae (Mut129 strain, 1 x 10^8CCU/mL, 50 µL/day for 3 days; n = 2 mice per group).
Description
Single-cell libraries were prepared using the 10x Genomics Chromium Single Cell 3′ Kit (v3.1) with a target capture of 6,000–8,000 cells per sample and sequenced on the Illumina NextSeq 500/550 platform.
The resulting dataset comprises processed cell-by-gene UMI expression matrices, UMAP dimensionality reduction coordinates, and cell cluster annotations derived using the Louvain algorithm (resolution = 0.8) and canonical cell markers to characterize local pulmonary immune heterogeneity during MP infection.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE342nnn/GSE342902 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE342902 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1509194 ncbi.nlm.nih.gov/bioproject/PRJNA1509194 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE342902 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |