Omics · study · 2026
Transport stress induces immune dysregulation and exacerbates Mycobacterium tuberculosis infection in mice
Listed in NCBI GEO
While transport stress (TS) is a well-known factor impacting immune function and infection, its cellular and molecular mechanisms remain elusive.
Description
In this study, we investigated the effects of TS on immune responses and its role in exacerbating Mycobacterium tuberculosis (Mtb) infection in mice. Behavioral assessments revealed that TS induced anxiety-like behaviors in mice.
Concomitantly, TS disrupted hypothalamic-pituitary-adrenal (HPA) axis homeostasis, altered hormone secretion patterns, and led to dysregulation of stress response genes (Egr1, Fos) and circadian regulators (Dbp, Per3). Single-cell RNA sequencing of the spleen revealed severe immune dysfunction, characterized by inhibition of Th17 differentiation, IL-17 signaling, and antigen presentation. Importantly, TS affects the crosstalk between the HPA axis and the spleen through ligand-receptor interactions, such as Pomc-Oprm1 and Gdf15-Tgfbr2.
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Furthermore, TS exacerbated the severity of Mtb infection, increased bacterial loads in the lungs and spleen, and attenuated infection-induced upregulation of immune genes (Tnfα, Il12b). Mechanistically, TS impairs adaptive immunity (Th1/Th2 differentiation) while amplifying neurotransmitter-related signaling, ultimately compromising host defenses. These findings highlight TS as a key contributor to neuroendocrine immune dysregulation, leading to increased susceptibility to infection.
Addressing stress-related immune dysfunction may be crucial for improving outcomes in tuberculosis and other infectious diseases.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE284nnn/GSE284562 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE284562 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1173960 ncbi.nlm.nih.gov/bioproject/PRJNA1173960 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE284562 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |