Lipidomics of human neutrophils during NET formation: PLD1-dependent phosphatidic acid and phosphoinositide remodelling

<p dir="ltr">Quantitative shotgun lipidomics of primary human neutrophils, underlying Figures 1 and 2 and Supplementary Figures 1 and 4 of Albarghash et al., "Phospholipase D1 activates NAD kinase fuelling neutrophil extracellular trap formation".</p><p dir="ltr">Four files are included; all values are pmol per sample, quantified against lipid class-specific internal standards.</p><p dir="ltr">Supplementary Data 1: PMA time course, lipid class totals.

Four conditions (unstimulated; PMA 30, 60 and 180 min) x 3 donors x 3 technical replicates. Underlies Figure 1A and the lipid class composition in Supplementary Figure 1B–E.</p><p dir="ltr">Supplementary Data 2: PMA time course, individual lipid species; the same 36 samples as Supplementary Data 1. Underlies Figure 1B–F.</p><p dir="ltr">Supplementary Data 3: PLD1 inhibitor experiment, individual lipid species.