Omics · study · 2026
Consequences of reactive astrogliosis in vivo IV
Listed in NCBI GEO
To investigate how molecularly induced reactive astrogliosis (MIRA) alters the transcriptome of the surrounding cells in the striatum, we locally injected AAV2/5 GfaABC1D-Cre-4X6T (MIRA) or AAV2/5 GfaABC1D-tdTomato (control) into the mouse striatum.
Description
Three weeks after AAV injection, fresh-frozen mouse striatal tissue was processed using the Chromium Nuclei Isolation Kit (10x Genomics, PN-1000494) according to the manufacturer's instructions.
Isolated nuclei were immediately subjected to single-nucleus RNA sequencing (snRNA-seq) library preparation using the Chromium GEM-X Single Cell 3′ Reagent Kit (10x Genomics, PN-1000686). Approximately 20,000 nuclei were loaded per sample, targeting recovery of ~15,000 nuclei. Libraries from eight samples were pooled and sequenced on an Illumina NovaSeq X Plus platform using half of a 25B flow-cell lane.
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Our analyses revealed that MIRA induces transcriptomic changes primarily in glial cells, with comparatively smaller effects in neurons, and is associated with increased immune-related gene expression in microglia.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336814 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336814 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1483395 ncbi.nlm.nih.gov/bioproject/PRJNA1483395 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE336814 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |