scRNA-seq of iPSC-derived dendritic cells under distinct differentiation and maturation protocols

To optimize differentiation protocols for generating functionally distinct DC subsets, we performed single-cell RNA sequencing (scRNA-seq) on iPSC-derived DCs cultured under various combinations of cytokine-driven differentiation and maturation conditions. We compared the effects of GM-CSF, IL-4, FLT3L, and different maturation cocktails including TNF-α, IL-1β, PGE2, and IFN-γ (mat2) or IFN-β (mat3).

The dataset captures transcriptional profiles of cells representing both monocyte-like and migratory dendritic cell populations. These data provide a valuable resource for understanding iPSC-DC heterogeneity and guiding the development of engineered DC-based therapies.