Type-I Interferon drives T-cell responses to amyloid-beta in the central nervous system

Spatial profiling was performed using the Molecular Cartography™ platform (Resolve Biosciences), an imaging-based highly multiplexed single-molecule fluorescence in situ hybridization (smFISH) technology.

The dataset includes raw transcript coordinate files, as well as cell segmentation outputs generated by the platform’s segmentation algorithm. Both segmentation-free (transcript-based) and segmentation-based spatial analyses were performed. These data were used to investigate age-dependent immune cell composition and spatial enrichment of T-cell phenotypes in amyloid plaque-associated regions, as described in the associated manuscript.