Single-cell RNA-seq profiling of brain myeloid cells following ischemic stroke in Ms4a3-specific Htr2b conditional knockout mice

However, the molecular mechanisms governing the functional heterogeneity of these populations remain poorly understood. In this study, we performed single-cell RNA sequencing (scRNA-seq) to characterize the cellular and transcriptional landscape of immune and non-immune cells in the ischemic brain using a myeloid-specific Htr2b conditional knockout model.

Htr2b^flox/flox mice and Htr2b^ERCre; Ms4a3-Cre mice were treated with tamoxifen to induce myeloid-lineage-specific recombination, followed by transient middle cerebral artery occlusion (tMCAO). Ipsilateral brain hemispheres were collected at 3 days post-stroke, a critical time point for inflammatory evolution and macrophage infiltration. Single-cell suspensions were generated from ischemic hemispheres, and both immune and non-immune cells were captured .