Omics · study · 2026
Single-nucleus transcriptomics reveals coordinated neuronal, immune, and glial dysregulation in the dorsolateral prefrontal cortex in major depressive disorder
Listed in NCBI GEO
We used single-nucleus RNA sequencing to define cell-type-specific molecular alterations in the dorsolateral prefrontal cortex (dlPFC) of individuals with MDD.
Description
We assessed cell-type composition, differential expression, pathway enrichment, oligodendrocyte-lineage pseudotime, ligand-receptor communication, transcription-factor regulon activity, and overlap with MDD-linked genetic variants. The integrated atlas resolved excitatory and inhibitory neuronal subclasses, oligodendrocyte-lineage cells, astrocytes, microglia/perivascular macrophages, endothelial cells, and vascular-associated populations.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE344nnn/GSE344216 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE344216 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1514828 ncbi.nlm.nih.gov/bioproject/PRJNA1514828 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE344216 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |