CCL Signaling Drives T Cell–Macrophage Crosstalk in the Mouse Colon During Chronic Trypanosoma cruzi Infection

The mechanisms underlying digestive Chagas disease (DCD), particularly within the colon, remain incompletely defined. To characterize the immune landscape during chronic infection, we used a murine model of DCD and performed single-cell RNA sequencing of colonic lamina propria cells.

Immune profiling revealed infiltration of T cells, B cells, and macrophages, with T cells representing the predominant immune population in the chronically infected colon. Computational cell–cell communication analysis predicted activation of chemokine signaling pathways, including the CCL5–CCR5 axis, mediating interactions between CD8+ T cells and macrophages. Processed single-cell gene expression matrices and associated metadata are provided in this submission