Omics · study · 2026
Impact of iPSC EVs on Immune Cells from Mouse Lymph Nodes
Listed in NCBI GEO
Extracellular vesicles (EVs) secreted by adult stem cells, particularly mesenchymal stem cells (MSCs), have garnered significant attention for their regenerative potential across various pathological conditions.
Description
However, despite this promise, the clinical translation of MSC EVs remains limited due to challenges such as donor variability and the finite proliferative capacity of MSCs, which pose significant obstacles to their therapeutic application.
In contrast, pluripotent stem cells (PSCs), including induced pluripotent stem cells (iPSCs), exhibit unlimited expansion potential when cultured under optimized conditions. This exceptional proliferative capacity enables the large-scale production of both PSCs and their EVs, making them highly attractive for therapeutic development. Additionally, embryos can achieve immune tolerance, yet the underlying mechanisms remain incompletely understood.
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We hypothesized that PSC EVs are enriched with a unique set of immunoregulatory molecules capable of modulating immune cells and promoting tolerance. To further explore the effects of iPSC EVs on immune cells within the lymph nodes, we performed single-cell RNA sequencing (scRNA-seq) using the 10x Genomics platform.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE291nnn/GSE291555 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE291555 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1234136 ncbi.nlm.nih.gov/bioproject/PRJNA1234136 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE291555 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |