Omics · study · 2026
A combinatorial transcription factor screening protocol for identifying cellular reprogramming minimal networks [scRNA-seq]
Listed in NCBI GEO
Direct reprogramming offers a powerful approach to generate therapeutic cell types, but progress is limited by an incomplete understanding of combinatorial transcription factor cooperativity.
Description
Here, we present a protocol for implementing REPROcode to identify reprogramming factor combinations that drive immune cell identities. This protocol details arrayed lentiviral production, transduction, and reprogramming of human fibroblasts into distinct immune cells.
We describe cell purification, library preparation, sequencing, and analysis to resolve transcription factor combinations and dynamics driving immune cell induction for immunotherapies.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE321nnn/GSE321717 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE321717 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1429326 ncbi.nlm.nih.gov/bioproject/PRJNA1429326 ↗
project · from NCBI GEO
- PubMed 42054212 pubmed.ncbi.nlm.nih.gov/42054212 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE321717 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |