Omics · study · 2026
Simultaneous analysis of leukemic mutations and transcriptomes at the single-cell level
Listed in NCBI GEO
Single-cell RNA-sequencing (scRNA-seq) enables detailed profiling of transcriptional heterogeneity in leukemic cells, but genetic variants are typically assessed by using separate assays, limiting the ability to link the genotype with the transcriptional state of a cell.
Description
We developed a method that enables the simultaneous genotyping and transcriptomic profiling of leukemic cells. We repurposed the intermediate barcoded full-length cDNA amplicons that are generated during the standard 3' 10X Genomics scRNA-seq workflow to selectively enrich for the transcripts of interest and subject them to PacBio long-read sequencing.
This approach enables the integrated analysis of somatic mutations and gene expression in the same cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329598 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329598 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1460300 ncbi.nlm.nih.gov/bioproject/PRJNA1460300 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Genome variation profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE329598 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:genome-variation-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |