Comparative Single Cell RNA Sequencing of Notch2 and RBPJ-deficient mononuclear phagocyte cells from the spleen

We employed single-cell RNA sequencing to investigate mononuclear phagocytic cell subsets, including splenic red pulp macrophages, monocytes, and dendritic cells in the spleen, with a focus on how Notch2 and its nuclear mediator Rbpj regulate these subsets. Our study offers a comparative analysis of splenic mononuclear phagocytes and specifically red pulp macrophages examining phagocytes isolated from myeloid Notch2 (N2ΔCx3cr1)-, and Rbpj (RbpjΔCx3cr1)-deficient mice or control animals.

Our findings may provide further insights into the heterogeneity and development of the splenic mononuclear phagocytic system including splenic macrophages and monocytes.