Supplemental Material for: High infiltration level of a prognostic improvement associated T cells is favorable for the prognosis of patients with gastric adenocarcinoma

We obtained scRNA-seq data of gastric cancer from the GEO database and used the Seurat package for clustering. We used the Bayesian cell proportion reconstruction algorithm to calculate the cell subpopulation content in TCGA samples, and combined it with TCGA survival data to perform Kaplan-Meier and univariable Cox analyses. We further performed multivariable Cox regression analysis incorporating age, sex, TNM stage, and histological type to evaluate the independent prognostic value of PIA T-cells.</p><p dir="ltr">Results: We found that a prognostic improvement associated T cells (PIA T-cells) had a better prognosis in gastric adenocarcinoma.

PIA T-cells expressed significantly higher levels of eight genes (ASPM, ATAD2, CENPF, DUT, MKI67, NUSAP1, TOP2A, and TYMS). Phenotypic characterization using canonical T-cell functional markers (CD4, CD8A, GZMB, IFNG, CD3D, CD3E, CD3G, and PDCD1) revealed a distinct phenotypic profile.</p><p dir="ltr">Conclusion: High infiltration of PIA T-cells is favorable for the prognosis of patients with gastric adenocarcinoma, and studying PIA T-cells may provide insights into treatments related to the gastric cancer tumor microenvironment.

The study of PIA T-cells may provide insights into gastric cancer tumor microenvironment-related treatments and inform future therapeutic strategies.</p>