Omics · study · 2026
Cellular composition of zebrafish spleen melanomacrophage centres
Listed in NCBI GEO
Antibody affinity maturation in mammals occurs in germinal centers (GCs), supported by follicular dendritic cells (FDCs) and T follicular helper (Tfh) cells.
Description
Teleost fish lack GCs, and their melanomacrophage centres (MMCs) have been proposed as functional analogues; however, the presence of FDCs remains uncertain. To define MMC cellular composition, we isolated autofluorescent MMCs from zebrafish spleens and performed single-cell RNA sequencing (scRNA-seq) on 13,632 cells.
Clustering and marker-based annotation identified centroblast-like and memory-like B cells, cytotoxic and memory CD8+ T cells, Th2/Tfh-like CD4+ T cells, and melanomacrophages with features of marginal zone and tingible body macrophages. Importantly, a progenitor fibroblastic reticular cell (pFRC)-like population expressed high levels of FDC-associated markers (mfge8, clu, vcam1, tnfsf13b, cxcl12), suggesting it may serve as an FDC analogue.
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Chemokine gene expression analysis highlighted a dominant CXCR4-CXCL12 axis, with pFRC-like cells expressing cxcl12 and ccl25 to potentially attract B and T cells, while lymphocyte subsets expressed corresponding receptors. Together, these data support a model in which melanomacrophages and pFRC-like cells mediate antigen presentation and B-cell selection, while Th2/Tfh-like cells provide survival signals. Thus, zebrafish MMCs meet the cellular requirements to enable affinity maturation, and define a primitive analogue of germinal centers
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313637 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313637 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1379687 ncbi.nlm.nih.gov/bioproject/PRJNA1379687 ↗
project · from NCBI GEO
- PubMed 42740903 pubmed.ncbi.nlm.nih.gov/42740903 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Danio rerio · Expression profiling by high throughput sequencing
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313637 | 7 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:7955 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |