Table · study · 2026
Modeling Stimulus-Induced Stress Responses in Microglia-like Cells Using a Commercial iPSC-dCas9-KRAB Line
Listed in NCBI GEO
This study investigates microglial responses to amyloid-beta (Aβ) and lipopolysaccharide (LPS) using human induced pluripotent stem cell (iPSC)-derived microglia-like cells (iMGLs). iMGLs were exposed to either Aβ or LPS and profiled by single-cell RNA sequencing (scRNA-seq) using the 10x Genomics platform.
Description
Transcriptomic analysis revealed both shared and stimulus-specific microglial activation states, characterized by inflammatory signaling, cell death pathways, and lipid metabolism.
In parallel, a CRISPR-based pooled survival screen was performed to identify genes critical for microglial survival. Guide RNA representation was measured at multiple differentiation time points (days 12, 24, 36, and 45) to infer gene essentiality during iMGL development. Together, these datasets provide insights into the molecular programs driving microglial stress responses and survival under neuroinflammatory conditions.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE306nnn/GSE306200 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE306200 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1309671 ncbi.nlm.nih.gov/bioproject/PRJNA1309671 ↗
project · from NCBI GEO
- PubMed 42214129 pubmed.ncbi.nlm.nih.gov/42214129 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Other
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE306200 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |