Synergistic degradation of circRNAs by RNase K and lysosomes

Compared to the other processes of circRNA metabolism, circRNA degradation is less understood. Through screening and further characterization, RNAseK and lysosome are identified as circRNA degradation modules in metazoan, and RNase 1 as a lysosomal circRNA endoribonuclease in mammal.

RNAseK and lysosome function synergistically with RNAseK degrading circRNAs outside and lysosome degrading those inside of the organelle. Mutations of RNAseK- or lysosome-sensitive sites in in vitro synthesized circRNA reporters lead to higher expression levels, and simultaneous mutations of both sensitive sites result in further increase. Under stress stimuli such as heat shock and ER stress, significantly decreased global circRNA levels are observed in C. elegans.