Omics · study · 2026
Single-cell RNA sequencing reveals acquisition of a FOXP3-positive regulatory-like state in relapsed TFH lymphoma
Listed in NCBI GEO
Nodal T follicular helper cell lymphomas (nTFHLs) are mature T-cell neoplasms characterized by transcriptional and phenotypic features of TFH cells.
Description
In this study, we analyzed relapsed TFH lymphoma samples using single-cell RNA sequencing, TCR repertoire analysis, whole-exome sequencing, and viral detection analysis. Single-cell transcriptomic profiling identified a dominant malignant T-cell population expressing canonical TFH-associated genes, with acquisition of a FOXP3-positive regulatory-like transcriptional state at relapse.
T-cell receptor clonotype analysis demonstrated oligoclonal expansion within the malignant compartment. Targeted genomic profiling identified recurrent TET2 mutations together with PLCG1 and TP53 alterations. Viral detection analysis identified EBV sequences but no HTLV-1/2 sequences.
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Processed Seurat objects, cell-level metadata, mutation annotation tables, and viral detection results are provided.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332984 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332984 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1469212 ncbi.nlm.nih.gov/bioproject/PRJNA1469212 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens · Other
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE332984 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[method].geo_series_type:other | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |