Omics · study · 2026
Role of E2F Transcription Factor 1 in the Pathogenesis of Pulmonary Arterial Hypertension. [Whole-lung single-cell RNA-seq]
Listed in NCBI GEO
Whole-lung single-cell RNA-seq was performed to define cellular and endothelial programs associated with E2F1 loss in the Egln1-driven pulmonary hypertension model.
Description
The manuscript analysis used whole-lung cell atlasing followed by computational endothelial subsetting for endothelial subtype, trajectory, pseudotime, and differential-expression analyses. The raw FASTQ files derive from one multiplexed 10x Genomics gene-expression library containing four probe-barcoded samples; this GEO submission includes the two demultiplexed samples used in the manuscript: Egln1_iCKO tagged with probe barcode BC001 and E2f1_iDKO tagged with probe barcode BC002.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335786 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335786 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1479450 ncbi.nlm.nih.gov/bioproject/PRJNA1479450 ↗
project · from NCBI GEO
- PubMed 42465492 pubmed.ncbi.nlm.nih.gov/42465492 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65% · Single-cell RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335786 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |