Circuit Organization and Transcriptomic Heterogeneity of Sympathetic Circuits Innervating Cranial Structures [scRNA-seq]

Coordinated activation of these targets is associated with the display of different emotions; however, the underlying circuit organization and cellular heterogeneity of SCG neurons remain unclear. Here, we combined neuronal tracing with single-cell and spatial transcriptomics to characterize the SCG circuitry and heterogeneity.

We found that each SCG neuron innervates a single effector organ. SCG subtypes defined by their projection (P) targets form two major compartments within the ganglia, but individual P-types were intermingled. Mature SCG transcriptomic types (T) emerge postnatally and exhibit rostra-caudal biases.