Omics · study · 2026
Restored clearance of senescent neutrophils by tissue-resident macrophages limits organ aging
Listed in NCBI GEO
Single-cell RNA sequencing of sorted mouse liver cells from young (6-8 month) and aged (23-25 month) Cx3cr1CreER;EP2wt/wt (WT) and Cx3cr1CreER;EP2lox/lox (EP2 cKO) male mice.
Description
Liver cells were enzymatically dissociated and live CD45+, CD1d+, or CD31+ cells were FACS-sorted in a 1:1:1 ratio, then processed using the 10x Genomics Chromium X platform (Next GEM Single Cell 3' Gene Expression). This dataset supports the finding that tissue-resident macrophage (TRM)-selective EP2 (PTGER2) deletion restores efferocytic clearance of senescent neutrophils and reverses age-associated transcriptional programs in Kupffer cells, preventing multi-organ aging phenotypes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE319nnn/GSE319489 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE319489 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1423603 ncbi.nlm.nih.gov/bioproject/PRJNA1423603 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE319489 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |