Omics · study · 2026
Single-cell transcriptomic profiling of c-Kit⁺ HSPCs in a KrasG12D/+ mouse model of JMML
Listed in NCBI GEO
We performed single-cell RNA sequencing (scRNA-seq) to characterize hematopoietic stem and progenitor cells (HSPCs) in a KrasG12D/+ mouse model of juvenile myelomonocytic leukemia (JMML).
Description
C-Kit⁺ HSPCs were enriched from the bone marrow of 6-week-old KrasG12D/+ and wild-type (WT) mice using CD117 MicroBeads and processed using the 10x Genomics Chromium Single Cell 3′ v3 platform. Libraries were sequenced on an Illumina NovaSeq 6000 system.
Raw data were processed with Cell Ranger and analyzed using Seurat for quality control, dimensionality reduction, and clustering. HSPC subsets, developmental trajectories, and pathway activities were then analyzed to characterize leukemic alterations.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE313nnn/GSE313553 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE313553 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1380309 ncbi.nlm.nih.gov/bioproject/PRJNA1380309 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE313553 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |