Omics · study · 2026
Single-cell transcriptomic landscape of ketone body–mediated modulation of renal mTORC1 signaling in proximal tubule–specific Tsc1 knockout mice
Listed in NCBI GEO
We performed single-cell RNA sequencing of mouse kidneys to investigate the effects of ketone bodies on renal mTORC1 signaling.
Description
A proximal tubule–specific Tsc1 knockout model (PTC- iKO) was used to induce constitutive mTORC1 activation. 1,3-butanediol, a precursor of ketone bodies that elevates circulating ketone levels, was administered as a dietary supplement. Three groups were analyzed: Tsc1 flox controls, Tsc1 PTC-iKO under a normal diet, and Tsc1 PTC-iKO with 1,3-butanediol treatment.
This dataset enables cell type–specific analysis of transcriptional changes associated with mTORC1 activation and ketone body exposure in the kidney.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328924 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328924 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1457028 ncbi.nlm.nih.gov/bioproject/PRJNA1457028 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE328924 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |