Single-Cell RNA Sequencing of the Human Endometrium in Healthy Controls and Patients with Polycystic Ovary Syndrome

However, the cellular and molecular heterogeneity of the endometrium in PCOS patients remains poorly understood. In this study, we performed single-cell RNA sequencing (scRNA-seq) on endometrial biopsies obtained from healthy control women and PCOS patients.

Endometrial samples were collected during the proliferative phase. We analyzed endometrial cell composition and transcriptional profiles at single-cell resolution. Our findings reveal PCOS-specific alterations in endometrial cell type composition, as well as cell-type-specific gene expression changes associated with inflammation, progesterone resistance, and extracellular matrix remodeling.