Omics · study · 2026
In vitro implantation model that recapitulates primary implantation events
Listed in NCBI GEO
We established an in vitro implantation model (IVIM) with mouse endometrial organoids (EMOs), mouse endometrial stromal cells (ESCs), and mouse blastocysts.
Description
EMOs expressing endometrial epithelial markers were cultured in Matrigel domes. In the IVIM, the outer surface of endometrial epithelial cells (EECs) faced the blastocyst within a microwell.
Under physiological conditions, blastocysts attach to the apical surface of EECs. Because the outer surface of EMOs cultured in Matrigel corresponds to the basal side, EMOs were retrieved from Matrigel and cultured for 4 days in an ultra-low attachment plate, inducing epithelial polarity inversion and generating apical-out EMOs. Microwells were fabricated in 7–8 mm glass-bottom wells by adding Matrigel containing ESCs and inserting a three-dimensional (3D) printed mold with multiple protrusions.
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After gelation at 37 °C, the mold was removed to form the microwells. Each microwell containing ESCs and an EMO was referred to as a mini-uterus, into which a blastocyst was placed and cultured using in vitro culture medium 2 (IVC2) medium. Confocal imaging demonstrated blastocyst invasion into the EMOs approximately 48–72 h after imaging began.
Single-cell RNA sequencing (scRNA-seq) was performed on cells recovered from the IVIM after 72 h of co-culture to characterize their cellular composition and transcriptional profiles.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335943 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335943 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1479856 ncbi.nlm.nih.gov/bioproject/PRJNA1479856 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Imaging 75% · RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335943 | 10 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:imaging | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |