Omics · study · 2026
A Single-Cell Transcriptomic Atlas of Human Lens Homeostasis and Aging
Listed in NCBI GEO
Lens epithelial cells (LECs) are crucial for lens transparency and function.
Description
Aging disrupts LEC homeostasis and contributes to the development of age-related cataracts (ARCs). The lens epithelium may rely on adult stem/progenitor cells to sustain homeostasis and counteract aging-related factors.
While the existence of stem cells in the lens epithelium has been supported by various studies, identifying these cells remains challenging. Recent advancements in single-cell RNA sequencing (scRNA-seq) provide high-resolution insights into cellular heterogeneity and offer a promising approach to elucidate the presence, origin, and age-related changes of lens stem/progenitor cells. We collected human donor lenses with ethical approval for scRNA-seq analysis, including samples from four non-aged (<65 years) and four aged (>65 years) individuals.
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After quality assessment and filtering, the transcriptome profiles of a total of 56,717 cells from two groups were depicted and merged to allow identification of overlapping cell populations in the following analysis. Subsequent analyses involved cell (sub)type characterization, trajectory inference, and cell-cell communication. Six types of lens superficial cells were identified by scRNA-seq, including four categories of LECs: early differentiation state LECs (eLECs), middle differentiation state LECs (mLECs), late differentiation state LECs (lLECs), and transient amplifying cells (TACs), along with a small number of lens fibre cells and immune cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE287nnn/GSE287919 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE287919 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1215283 ncbi.nlm.nih.gov/bioproject/PRJNA1215283 ↗
project · from NCBI GEO
- PubMed 40598599 pubmed.ncbi.nlm.nih.gov/40598599 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE287919 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |