Omics · study · 2026
An Intra-BET Epigenetic Mechanism Driven by BRD4 Controls Pancreatic α-cell Expansion [scRNA-seq]
Listed in NCBI GEO
This study investigates the role of BET family member BRD4 in pancreatic islet organoid differentiation and endocrine cell development.
Description
Single-cell RNA sequencing was performed to characterize transcriptomic changes across three experimental systems: (1) human islet organoids following adenovirus-mediated BRD4 overexpression (B4OV) versus control; (2) pancreatic islets from α-cell-specific Brd4 knockout (αKO) mice versus control mice; and (3) stage 5 pancreatic endocrine progenitors following combined BRD4 activation and BRD2 suppression (B2sB4) versus control, after differentiation to stage 6.
The dataset enables identification of BRD4-regulated gene expression programs and cellular heterogeneity during pancreatic endocrine differentiation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE346nnn/GSE346272 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE346272 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1524262 ncbi.nlm.nih.gov/bioproject/PRJNA1524262 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75% · Single-cell RNA sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE346272 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |