Omics · dataset · 2026
DataSheet1_IDEIS: a tool to identify PTPRC/CD45 isoforms from single-cell transcriptomic data.pdf
Listed in HKU DataHub and figshare and Loughborough Research Repository and UP Research Data Repository — shown once because both records carry DOI 10.3389/fimmu.2024.1446931.s001
<p>Single-cell RNA sequencing (scRNA-seq) methods are widely used in life sciences, including immunology.
Description
Typical scRNA-seq analysis pipelines quantify the abundance of particular transcripts without accounting for alternative splicing. However, a well-established pan-leukocyte surface marker, CD45, encoded by the PTPRC gene, presents alternatively spliced variants that define different immune cell subsets.
Information about some of the splicing patterns in particular cells in the scRNA-seq data can be obtained using isotype-specific DNA oligo-tagged anti-CD45 antibodies. However, this requires generation of an additional sequencing DNA library. Here, we present IDEIS, an easy-to-use software for CD45 isoform quantification that uses single-cell transcriptomic data as the input.
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We showed that IDEIS accurately identifies canonical human CD45 isoforms in datasets generated by 10× Genomics 5’ sequencing assays. Moreover, we used IDEIS to determine the specificity of the Ptprc splicing pattern in mouse leukocyte subsets.</p>
Links
Where it is published
- DOI doi.org/10.3389/fimmu.2024.1446931.s001 ↗
DOI / persistent id · from datahub hku hk
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from datahub hku hk
Topics
- From keywords
- Animal immunology · Animal immunology · Animal immunology · Animal immunology · Astronomy & Astrophysics · Autoimmunity · Autoimmunity · Autoimmunity · Autoimmunity · Cellular immunology · Cellular immunology · Cellular immunology · Cellular immunology · Chemistry · Chemistry · Computer Science & AI · Computer Science & AI · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Economics & Finance · Economics & Finance · Engineering · Engineering · Engineering · Engineering · Gene expression · Gene expression · Gene expression · Gene expression · Humanities · Humanities · Humanities · Humoural immunology and immunochemistry · Humoural immunology and immunochemistry · Humoural immunology and immunochemistry · Humoural immunology and immunochemistry · Immunology · Immunology · Immunology · Immunology · Innate immunity · Innate immunity · Innate immunity · Innate immunity · Life Sciences · Life Sciences · Life Sciences · Life Sciences · Materials Science · Mathematics & Statistics · Medicine & Health · Medicine & Health · Ocean & Atmospheric Science · Psychology & Behavioral Science · Single-cell RNA sequencing · Single-cell RNA sequencing · Single-cell RNA sequencing · Single-cell RNA sequencing · Social Science · Social Science · Social Science · Transplantation immunology · Transplantation immunology · Transplantation immunology · Transplantation immunology · Tumour immunology · Tumour immunology · Tumour immunology · Tumour immunology · Veterinary immunology · Veterinary immunology · Veterinary immunology · Veterinary immunology
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 4 source records, 82 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| HKU DataHub | oai:figshare.com:article/34014438 | 6 d ago | JSON v1 |
| figshare | oai:figshare.com:article/34014438 | 5 d ago | JSON v1 |
| Loughborough Research Repository | oai:figshare.com:article/34014438 | 5 d ago | JSON v1 |
| UP Research Data Repository | oai:figshare.com:article/34014438 | 5 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].anzsrc:field:300906 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Veterinary Immunology'] |
| concepts[field].anzsrc:field:300906 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Veterinary Immunology'] |
| concepts[field].anzsrc:field:300906 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Veterinary Immunology'] |
| concepts[field].anzsrc:field:300906 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Veterinary Immunology'] |
| concepts[field].anzsrc:field:310505 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['gene expression'] |
| concepts[field].anzsrc:field:310505 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['gene expression'] |
| concepts[field].anzsrc:field:310505 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['gene expression'] |
| concepts[field].anzsrc:field:310505 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['gene expression'] |
| concepts[field].anzsrc:field:310905 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Animal Immunology'] |
| concepts[field].anzsrc:field:310905 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Animal Immunology'] |
| concepts[field].anzsrc:field:310905 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Animal Immunology'] |
| concepts[field].anzsrc:field:310905 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Animal Immunology'] |
| concepts[field].anzsrc:field:320403 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Autoimmunity'] |
| concepts[field].anzsrc:field:320403 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Autoimmunity'] |
| concepts[field].anzsrc:field:320403 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Autoimmunity'] |
| concepts[field].anzsrc:field:320403 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Autoimmunity'] |
| concepts[field].anzsrc:field:320404 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Cellular Immunology'] |
| concepts[field].anzsrc:field:320404 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Cellular Immunology'] |
| concepts[field].anzsrc:field:320404 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Cellular Immunology'] |
| concepts[field].anzsrc:field:320404 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Cellular Immunology'] |
| concepts[field].anzsrc:field:320405 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Humoural Immunology and Immunochemistry'] |
| concepts[field].anzsrc:field:320405 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Humoural Immunology and Immunochemistry'] |
| concepts[field].anzsrc:field:320405 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Humoural Immunology and Immunochemistry'] |
| concepts[field].anzsrc:field:320405 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Humoural Immunology and Immunochemistry'] |
| concepts[field].anzsrc:field:320407 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Innate Immunity'] |
| concepts[field].anzsrc:field:320407 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Innate Immunity'] |
| concepts[field].anzsrc:field:320407 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Innate Immunity'] |
| concepts[field].anzsrc:field:320407 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Innate Immunity'] |
| concepts[field].anzsrc:field:320408 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Transplantation Immunology'] |
| concepts[field].anzsrc:field:320408 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Transplantation Immunology'] |
| concepts[field].anzsrc:field:320408 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Transplantation Immunology'] |
| concepts[field].anzsrc:field:320408 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Transplantation Immunology'] |
| concepts[field].anzsrc:field:320409 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Tumour Immunology'] |
| concepts[field].anzsrc:field:320409 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Tumour Immunology'] |
| concepts[field].anzsrc:field:320409 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Tumour Immunology'] |
| concepts[field].anzsrc:field:320409 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Tumour Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].anzsrc:group:3204 | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['Immunology'] |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:humanities | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:materials-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:mathematics-statistics | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:psychology-behavioral | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[field].local:field:social-science | mapping · researchdata up ac za | connector:researchdata_up_ac_za@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · repository lboro ac uk | connector:repository_lboro_ac_uk@1.0.0 | |
| concepts[modality].local:modality:rna-seq | enrichment · datahub hku hk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · datahub hku hk | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:single-cell-rna-seq | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['single-cell RNA sequencing'] |
| concepts[modality].local:modality:single-cell-rna-seq | mapping · datahub hku hk | vocabulary-mapper@1.0.0 | keywords['single-cell RNA sequencing'] |
| concepts[modality].local:modality:single-cell-rna-seq | mapping · researchdata up ac za | vocabulary-mapper@1.0.0 | keywords['single-cell RNA sequencing'] |
| concepts[modality].local:modality:single-cell-rna-seq | mapping · repository lboro ac uk | vocabulary-mapper@1.0.0 | keywords['single-cell RNA sequencing'] |
| description | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | /metadata/dc/description |
| license | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | /metadata/dc/rights |
| publication_date | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | |
| title | source · datahub hku hk | connector:datahub_hku_hk@1.0.0 | /metadata/dc/title |