14,068 datasets

NCBI GEO2026 · Excel spreadsheet · 14 samples

We identified 5 patients from two families with a defect in antibody production without a known inborn error of immunity.

NCBI GEO2026 · Excel spreadsheet · 35 samples

People living with HIV (PLWH) on antiretroviral therapy (ART) accumulate proviral sequences in genomes of often clonally expanded CD4+ HIV-1 target cells. Viral-derived DNA is preferentially found at distinct genomic loci suggesting a selective process driven by integration site-specific crosstalk between viral and host sequences. Focusing on one of the most prominent selected integration loci, th

NCBI GEO2026 · RNA sequencing · 9 samples

This work explores tissue-specific biomimetic functional materials and investigates the regulatory mechanisms of materials on cells, offering a highly promising solution for the manufacturing of centimeter-sized injectable liver tissue.

NCBI GEO2026 · RNA sequencing · 18 samples

DNA methylation regulates transcription, yet the demethylation of canonical elements like promoter CpG islands exhibits inconsistent correlations with gene activation. We hypothesized that causal regulatory elements are defined by biophysical hypersensitivity. Profiling 24 whole-genome bisulfite sequencing samples across diverse human and murine models, we identified Methylation Mesa, narrow (~45-

NCBI GEO2026 · RNA sequencing · 6 samples

Spermatogonial stem cells (SSCs) are required for initiating and maintaining normal spermatogenesis, and notably, they have significant applications in both reproductive and regenerative medicine owing to their great plasticity with de-differentiation and trans-differentiation potentials. Nevertheless, molecular mechanisms regulating human SSC fate determinations and male fertility remain elusive.

NCBI GEO2026 · RNA sequencing · 204 samples

RNA sequencing was performed on human stem cell-derived neuronal cultures across developmental and experimental conditions, including differentiated neurons exposed for 24 h to the mitochondrial complex I inhibitor rotenone at 0.06, 0.25, 0.5 or 1 µM. The transcriptomic data were generated to support characterisation of human dopaminergic neuronal metabolism and integration with quantitative exome

NCBI GEO2026 · RNA sequencing · 14 samples

Impaired alveolar regeneration underlies numerous chronic lung diseases, yet the metabolic determinants governing this process remain poorly understood. Effective alveolar repair depends on the differentiation of type 2 alveolar epithelial cells (AT2), the resident lung stem/progenitor cells, into type 1 alveolar epithelial cells (AT1) to restore gas exchange surfaces. Here, we reveal that glutami

NCBI GEO2026 · RNA sequencing

We compared gene expression of aged and young oligodendrocyte progenitor cells (OPC) and identified differentially expressed genes.

NCBI GEO2026 · RNA sequencing · 2 samples

As direct experimentation on the healthy human brain is ethically limited, developing brain models is essential. Currently, research groups employ complex in vitro brain organoid models, three-dimensional (3D) structures that mimic aspects of the human brain architecture. These organoids self-organize into small clusters that resemble key features of the developing brain, including functional neur

NCBI GEO2026 · RNA sequencing · 12 samples

Introduction: Macrophages are essential components of the innate immunity, serving as a frontline defense against pathogens and contributing to tissue homeostasis. The generation of human induced pluripotent stem cell (iPSC)-derived macrophages (iMacs) provides a valuable platform for studying human innate immunity and macrophage biology. This study presents a robust, reproducible, and efficient p

NCBI GEO2026 · RNA sequencing · 12 samples

In young animals, oligodendrocyte progenitor cells (OPCs) undergo robust differentiation, progressing through stages to become pre-myelinating oligodendrocytes (Pre-OL) and ultimately myelinating oligodendrocytes (OLs). However, OPCs in aged animals are limited in their differentiation ability. This disrupts myelin maintenance in the central nervous system (CNS), leading to a lack of remyelination

NCBI GEO2026 · RNA sequencing · 14 samples

Apolipoprotein E4 (APOE4), the strongest genetic risk factor for late-onset Alzheimer's disease, promotes neuronal dysfunction through incompletely understood mechanisms. Here, we integrated transcriptomic, translatomic, and proteomic profiling of isogenic human iPSC-derived APOE3 and APOE4 neurons and found that APOE4 fundamentally impairs neuronal proteome renewal. Although transcriptional chang

NCBI GEO2026 · RNA sequencing · 60 samples

This SuperSeries comprises transcriptomic and epigenomic datasets generated in human macrophages to investigate IL-6-STAT3 signaling. The datasets include RNA-seq under STAT3 degradation and JAK inhibition, IL-6 time-course RNA-seq, hMDM RNA-seq, ATAC-seq, and CUT&Tag profiling of H3K27ac, STAT3, and STAT1. Together, these data characterize IL-6-induced chromatin and transcriptional changes and th

NCBI GEO2026 · RNA sequencing · 50 samples

This SuperSeries is composed of the SubSeries listed below.

NCBI GEO2026 · RNA sequencing · 46 samples

Shortly after mitosis, a wave of hyper-transcription reactivates the genome, especially in mouse embryonic stem (ES) cells, where rapid reactivation is essential for self-renewal and pluripotency. While recent work has illuminated how specific groups of genes are reactivated, whether dedicated mechanisms enable the global, efficient and accurate post-mitotic reactivation of the genome remains unkn

NCBI GEO2026 · RNA sequencing · 2 samples

We performed single-cell RNA sequencing of the corallimorpharian Ricordea yuma and the stony coral Galaxea fascicularis to characterize their cell-type diversity and compare cell-type evolution between corallimorpharians and scleractinian corals.

NCBI GEO2026 · RNA sequencing · 9 samples

Proline/arginine-rich end leucine-rich repeat protein (PRELP) is an extracellular matrix-associated protein involved in tissue remodeling and immune regulation. This study investigates PRELP-associated transcriptional changes in human melanoma using bulk RNA sequencing of PRELP-low, PRELP-high, and PRELP + Toll-like receptor 2 (TLR2) co-transfected BUF1088 cells. PRELP-high cells were generated by

NCBI GEO2026 · RNA sequencing · 25 samples

Efforts to analyze breast cancer transcriptomes at scale have been limited by cross-study technical variability. To address this, we adapted single cell RNA sequencing analytical strategies to harmonize 7,612 bulk breast cancer transcriptomes from 22 datasets, generating a high resolution atlas that resolves both known and previously unrecognized malignant transcriptional states. This framework id

NCBI GEO2026 · RNA sequencing · 2 samples

Purkinje cells are specialized cardiomyocytes coordinating rapid electrical propagation for synchronized heart contraction. Although these cells were described over a century ago, their molecular and cellular features under physiological conditions and after myocardial infarction are not fully understood. Here we show that single nucleus transcriptome sequencing identifies three types of Purkinje

NCBI GEO2026 · RNA sequencing · 12 samples

Interleukin-6 (IL-6) is a pleiotropic cytokine that drives macrophage functions ranging from tissue homeostasis to inflammation, yet how STAT3 specifies these divergent outcomes remains unclear. By integrating transcriptomic and epigenomic profiling with selective STAT3 degradation, we show that STAT3 controls IL-6-induced epigenomic remodeling and restrains a latent STAT1-associated interferon pr

NCBI GEO2026 · RNA sequencing · 4 samples

A novel method for the efficient construction and rapid vascularization of functional hepatocyte aggregates in vitro has been developed in this research.

NCBI GEO2026 · RNA sequencing

Developmental transitions involve changes in the genetic programs governing molecular processes. Characterizing these genetic programs gives us insight into how genes work to orchestrate developmental transitions in morphology, physiology, or even behavior. One fascinating developmental transition, metamorphosis, bridges larval and juvenile periods. Southern Flounder, a flatfish, is an ideal model

NCBI GEO2026 · Excel spreadsheet · 8 samples

We performed bulk RNA sequencing to characterize temporal transcriptional dynamics during developmentally guided differentiation of human embryoic stem cells (hESCs) into ventral midbrain dopaminergic (vmDA) lineage cells. Samples were collected at four defined stages: undifferentiated hESCs (DIV 0), early patterned progenitors (DIV 9), late vmDA progenitors (DIV 16), and maturing vmDA neurons (DI

NCBI GEO2026 · RNA sequencing · 38 samples

Cardiovascular disease (CVD) etiology is strongly influenced by lead (Pb) exposure, but the underlying molecular and functional mechanisms are unclear, particularly during development. Using human induced pluripotent stem cell (iPSC) derived cardiomyocytes, we examined the effects of human-relevant Pb exposure during ventricular cardiomyocyte differentiation on transcription at several time points

NCBI GEO2026 · RNA sequencing · 9 samples

We performed a high throughput screen of a newly curated covalent fragment library comprising 894 compounds. The phenotypic screen was designed to identify signalling pathway modulators and constituted a differentiation assay whereby primed human induced pluripotent cells (iPSCs) were differentiated to definitive endoderm over a period of 4 days in the presence of DMSO (control) or the individual