384 datasets

NCBI GEO2026 · RNA sequencing · 25 samples

Astrocytes play a pivotal role in maintaining brain homeostasis. In response to injury or disease, functional changes in astrocytes can be detrimental to neural repair and contribute to the progression of neuroinflammatory conditions. While studies have characterized the gene expression programs activated in astrocytes during neuroinflammation, the gene regulatory mechanisms underlying proinflamma

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 18 samples

The association between diseases and microbiota has recently received considerable attention in several fields. Extracellular vesicles (EVs) are widely known as communication tools, and are secreted by several types of cells. Recent studies have revealed that both gram-positive and -negative bacteria secrete EVs. In this study, we investigated the bactrial-derived EVs in vitro.

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 6 samples

ELEs orchestrate a multi-dimensional reversal of replicative senescence in 3D skin models through telomere protection, replication stress alleviation, inflammation suppression, and ECM homeostasis remodeling. These effects are mechanistically coordinated by HES1-mediated stemness reprogramming and the concerted modulation of MAPK signaling (particularly the ERK cascade), PI3K/AKT, and cAMP signali

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 8 samples

Postoperative cognitive dysfunction (POCD) commonly affects elderly surgical patients and produces declines in memory, attention, and executive function that substantially reduce quality of life. The precise pathogenesis of POCD remains unclear. Recent studies implicate trauma-induced epigenetic mechanisms in its development. Conventional sequencing methods are hindered by reverse-transcription ar

NCBI GEO2026 · Excel spreadsheet · 6 samples

Small regulatory RNAs (sRNAs) serve as key drivers of bacterial adaptation to environmental fluctuations. They critically control the adaptive response of Staphylococcus aureus and other pathogens to iron and manganese restriction imposed by the host. However, it is unknown if bacterial sRNAs enable adaptation to other metal-limited environments. This study examined the repertoire of sRNAs produce

NCBI GEO2026 · Excel spreadsheet · 48 samples

This study investigated viral- and host-encoded miRNAs in serum from persons with (PwMS) or without (PwoMS) MS with the goal of identifying miRNAs associated with MS phenotypes and responses to therapy. PwMS were stratified by Low- and High-EDSS and compared to PwoMS in Discovery (DC) and Validation (VC) Cohorts. miRNAs were measured by sequencing in the DC that were verified by droplet digital PC

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 6 samples

Small extracellular vesicles (sEVs) derived from human skeletal muscle cells (HSkMCs) at different stages of myogenic differentiation were subjected to small RNA sequencing to characterize changes in sEV-associated miRNA profiles. Comparative analysis identified differentially expressed miRNAs during myogenic differentiation, including miR-193b-3p, which was enriched in sEVs derived from different

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 3 samples

Small RNA-seq was performed to investigate the impact of piRNAs on pluripotency during embryonic germ cells and in germline stem (GS) cells. The dataset includes PIWIL2-bound small RNAs isolated from E16 mouse testes and small RNAs from Piwil4 KO GS cells.

NCBI GEO2026 · RNA sequencing · 20 samples

Non-coding RNAs play a significant role in embryonic development, but their contribution to the pathogenesis of human intestinal diseases remains unclear. We utilized high-throughput sequencing technology to reveal the regulatory mechanisms that affect gene expression during human intestinal development. These results contribute to a deeper understanding of the role of non-coding RNAs in human int

NCBI GEO2026 · Excel spreadsheet · 6 samples

Psoriasis is an immune-mediated chronic inflammatory skin disease, which is clinically characterized by erythema and scales. The pathogenesis is complex, easy to relapse and difficult to cure. Mesenchymal stem cell exosomes (MSCs) are well known for their potent immunomodulatory properties. However, the possible role and underlying mechanisms of adipose mesenchymal stem cell exosomes (ADSC-EVs) in

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 9 samples

The present study identified and characterized miRNAs, which may play a major role in stress resistance. we applied high-throughput sequencing to investigate the alterations of exosomal miRNAs expression of sea cucumber under hypoixa stress (DO 2mg/L) last for three days (LO3-1,LO3-2,LO3-3) and seven days (LO7-1, LO7-2, LO7-3), and normal condition(NC-1, NC-2, NC-3). These results will provide a b

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 4 samples

The goal is to find out the miRNA expression changes when RBM34 was Knocked down and overexpressed.

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 6 samples

Aim of the study: Cannabinoids have gained attention for their ability to modulate immune responses and suppress inflammation. However, the effect of Δ⁸-THC, a minor cannabinoid found in Cannabis, has not been explored in autoimmune hepatitis. To address knowledge gap, the present study aimed to investigate the hepatoprotective and immunoregulatory properties of Δ⁸-THC in experimental autoimmune h

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 6 samples

Islet inflammation is a hallmark of both Type 1 and Type 2 diabetes. To have a global view, we performed small non-coding RNAs profiling on mouse islets exposed for 24h to a mix of pro-inflammatory cytokines. We analyzed the impact on isomiR levels and observed several canonical miRNAs and isomiRs that are deregulated in inflammatory conditions.

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 36 samples

Transposable elements (TEs) are mobile DNA sequences that can reshape genomes, yet their role in crop domestication and adaptation has not been systematically investigated. Here we show that TE-associated genetic variants drive heritable epigenetic changes underlying key domestication traits in foxtail millet (Setaria italica), a cereal domesticated from green millet within the past ~11,000 years.

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 8 samples

Aging is accompanied by functional decline and increased senescence of pancreatic β-cells. These changes may be influenced by islet-resident macrophages (iMACs) that remodel tissue in response to environmental cues. To explore the molecular mechanisms underlying β-cell aging and senescence, we profiled small non-coding RNAs (sncRNAs) in FACS-sorted β-cells and iMACs from 3-, 12-, and 22-month-old

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 12 samples

Aging is accompanied by functional decline and increased senescence of pancreatic β-cells. These changes may be influenced by islet-resident macrophages (iMACs) that remodel tissue in response to environmental cues. To explore the molecular mechanisms underlying β-cell aging and senescence, we profiled small non-coding RNAs (sncRNAs) in FACS-sorted β-cells and iMACs from 3-, 12-, and 22-month-old

NCBI GEO2026 · Excel spreadsheet · 6 samples

Aging is accompanied by functional decline and increased senescence of pancreatic β-cells. These changes may be influenced by islet-resident macrophages (iMACs) that remodel tissue in response to environmental cues. To explore the molecular mechanisms underlying β-cell aging and senescence, we profiled small non-coding RNAs (sncRNAs) in FACS-sorted β-cells and iMACs from 3- and 12-month-old mouse

NCBI GEO2026 · Excel spreadsheet · 6 samples

Aging is accompanied by functional decline and increased senescence of pancreatic β-cells. These changes may be influenced by islet-resident macrophages (iMACs) that remodel tissue in response to environmental cues. To explore the molecular mechanisms underlying β-cell aging and senescence, we profiled small non-coding RNAs (sncRNAs) in FACS-sorted β-cells and iMACs from 3- and 12-month-old mouse

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 27 samples

Urinary toxicities are common following prostate radiotherapy and can significantly impact quality of life. There is a need for biomarkers that can predict the risk of urinary toxicity following treatment using MRI guided stereotactic body radiotherapy (MR-SBRT) to assist with selection of the most appropriate radiotherapy treatment approach and to potentially provide early intervention for toxici

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 12 samples

Aging is accompanied by functional decline and increased senescence of pancreatic β-cells. These changes may be influenced by islet-resident macrophages (iMACs) that remodel tissue in response to environmental cues. To explore the molecular mechanisms underlying β-cell aging and senescence, we profiled small non-coding RNAs (sncRNAs) in FACS-sorted β-cells and iMACs from 3-, 12-, and 22-month-old

NCBI GEO2026 · Excel spreadsheet · 12 samples

Brain aging is not an independent process, yet how systemic aging drives neural decline remains unclear. Here, we identified a circulating miR-4433b-3p, packaged within extracellular vesicles (EVs), as a trans-organ effector bridging cardiac aging with central nervous system (CNS) decline. Small RNA sequencing and human cohort validation revealed selective enrichment of miR-4433b-3p in aged plasma

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 9 samples

The Microprocessor complex, minimally composed of DROSHA and DGCR8, is ubiquitously required for microRNA biogenesis. It also regulates the balance between pluripotency and differentiation through mRNA alternative splicing and mRNA cleavage. However, how these various activities are controlled remains unclear. Both DROSHA and DGCR8 are phosphoproteins, and phosphorylation has been reported to infl

NCBI GEO2026 · RNA sequencing · 15 samples

Small nucleolar RNAs (snoRNAs) are structured non-coding RNAs that are frequently under-quantified by standard RNA-seq protocols, since their compact secondary structure impedes efficient reverse transcription. TGIRT-seq relies on a thermostable group II intron reverse transcriptase with high fidelity, processivity, and structure-tolerant activity, making it particularly well suited to accurately

NCBI GEO2026 · Non-coding RNA profiling by high throughput sequencing · 60 samples

Small-RNA sequencing of a plasma-derived, GLAST-positive small extracellular-vesicle fraction was performed in a discovery cohort of 60 participants, including 45 participants with amyotrophic lateral sclerosis (ALS) and 15 non-neurological controls. One small-RNA sequencing library was generated per participant using the QIAseq miRNA Library Kit and sequenced on an Illumina NovaSeq 6000 at the Ya